Best to start off with 3, then if 1 does die, then the others won't be lonely. If you do ever get to the stage where you do have just 1 left, she'll be fine for a couple of days or so, but I'd then introduce another 2. That way, any possible aggro from the first hen (she'll be top of the pecking order) will have to be divided. Introduce new girls at night, pop them into the coop at bedtime, shut the door and they'll (mostly) be fine
Either that or completely change the coop and run location so that it's all new for all of them

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